Allison Baumhover - Animal Science
Effects of Cu Status on Total Tract Gut Permeability in Dairy x Beef Crossbred Steers
Project Advisor(s): Stephanie Hansen | Advisor(s): Rodrigo Tarte
Abstract: Dairy calves, however, are exposed to higher concentrations of copper (Cu) during gestation than native beef calves due to management practices in the dairy industry resulting in elevated Cu status upon entering the feedlot. Excessive copper accumulation in the liver causes oxidative stress and interferes with important cellular functions which may lead to immune dysfunction and the degradation of epithelial cell junctions – commonly known as “leaky gut”. CrEDTA, an indigestible marker, is too large to pass through healthy epithelial cell junctions and will only be found in the bloodstream of compromised individuals. Therefore, the aim of this investigation was to assess the influence of liver Cu status on the epithelial integrity of dairy x beef steers utilizing CrEDTA. After liver samples were analyzed, steers were sorted into a High and a Low treatment based on liver Cu concentration. On day –1 the steers were trucked for 6 hours before unloading at the Animal Resource Station (Iowa State University) in Ames, Iowa. Steers were separated in two separate pens based on treatment group. On day 0 and day 7, calves were orally administered 500 mL of CrEDTA at hour 0. Jugular blood samples were collected at hour 4.5 on average for subsequent plasma analysis. Plasma Cr concentrations did not differ due to Cu status (Low vs High) on either day 0, representing the post transit period, or day 7, representing peak disease period. While no statistically significant differences were observed between the two treatments, it is important to note that a numerical distinction on day 0, steers on the Low treatment exhibited slightly lower levels of plasma Cr compared to their counterparts in the High treatment. It is crucial to recognize, like most biological processes, that mineral status is just a piece of the puzzle. The complex interplay between Cu, rumen health, acidosis, and illness all contribute to leaky gut.
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Emma Bock – Biology (AGLS)
The Effectiveness of Plant Compounds in Inhibiting Bacterial Growth in Chicken Fecal Samples
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Project Advisor(s): Qijing Zhang | Advisor(s): Em Kapler
Abstract: Campylobacter jejuni is a foodborne bacterial pathogen that causes gastrointestinal illness in humans and abortion in ruminants. Due to its clinical significance and rising resistance to antibiotics, Campylobacter is a major public health concern. Hence, alternatives to antibiotics are needed for effective control of Campylobacter. Cryptolepine is derived from a West African plant and has antimicrobial properties, while carnosic acid is derived from common rosemary and Salvia plant extracts and has been shown to have antibacterial activity. However, it is unclear if cryptolepine and carnosic acid are effective against Campylobacter. The purpose of this study was to determine whether cryptolepine and carnosic acid inhibited Campylobacter under conditions simulating in vivo infection. When added into fecal samples collected from chickens experimentally colonized with Campylobacter, both cryptolepine or carnosic acid produced significant reduction in viable cell counts in a dose-dependent manner. We also measured the killing curve of carnosic acid over a 24-hour period. At twice the MIC concentration and treated for 8 hours, carnosic acid completely killed Campylobacter, while the non-treated control had an increase in viable cell counts, indicating carnosic acid is bactericidal. These results demonstrate the anti-Campylobacter activity of cryptolepine and carnosic acid and their potential as antimicrobial alternatives.
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Darcy Bonds - Horticulture
Exploring the Genetic Diversity of Genes Involved in Plant Fatty Acid Elongation across a Diverse set of Maize Inbred Lines
Project Advisor(s): Marna Yandeau-Nelson | Advisor(s): Cynthia Haynes
Abstract: The cuticle produced by the plant epidermis performs many essential functions. The cuticle is comprised of a cutin matrix that is infused with and laid atop by cuticular waxes. The precursors of cuticular waxes are very long chain fatty (VLCF) acyl-CoAs, which are produced in the Fatty Acid Elongation pathway, in which the 3-ketoacyl-CoA (KCS) enzyme is active. This family shows a significant amount of genetic redundancy, possessing 26 unique KCS-encoding genes in the maize inbred B73 genome. In this study, we examine the genetic diversity of KCS genes across 26 genetically diverse inbred maize lines that are the parental founders of the Maize Community’s Nested Association Mapping (NAM) as well as two additional inbred lines Mo17 and W22. Importantly these maize inbred lines have been sequenced and exhibit structural variations when comparing across genomes, including the presence/absence of gene sequences. The objective of this study is to assess whether the KCS gene family is dynamic in gene number across inbred lines and the breadth of genetic diversity that exists among these genes. This work will shed light on the genetic diversity of KCS genes and provide the foundation for studying potential diversity in function across the gene family.
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Sydney Cuddeback – Agricultural Business, Finance
Succession Planning for Farmers
Project Advisor(s): Michael Bootsma | Advisor(s): Darin Wohlgemuth, Rhaechel Ohge Fritz
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Mason Erger – Biology (AGLS)
Researching a Novel Biosynthesis for GHB
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Project Advisor(s): Scott Nelson | Advisor(s): Em Kapler
Abstract: Several months ago, I discovered a series of internet posts and research articles suggesting novel biosynthesis for the date-rape drug Gamma-Hydroxybutyric Acid, or GHB. Having known several people who have suffered from sexual abuse in their life, this novel biosynthesis was alarming to me. Particularly because the precursor chemicals are easily accessible to the general public, and the synthesis could be performed with no risk of detection in a college dorm room anywhere on ISU’s campus. I arranged to perform a series of experiments to prove or disprove the validity of this synthesis, as it had never been formally performed in a laboratory setting. I utilized a series of different media to grow the organism supposedly able to produce the compound, S. cerevisiae, and provided it with the substrate that supposedly allowed it to do so, Monosodium Glutamate. After several weeks of testing and recording growth speeds, it became apparent that MSG inhibited the growth of S. cerevisiae and furthermore, that S. cerevisiae was incapable of producing GHB in any of the conditions I provided to it.
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Claire Holtkamp – Global Resource Systems, Chemistry
Organophosphates and Epilepsy: Evaluating Toxicity of Nanoparticles used in Epilepsy Treatment
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Project Advisor(s): Thimmasettapp Thippeswamy, Christina Meyer, Dorothy Masinde | Advisor(s): Amanda Chung, Benjamin Noack
Abstract: This study delves into the pressing issue of organophosphate (OP)-induced epilepsy, a prevalent neurological disorder resulting from acute OP exposure. OPs are widely used in pesticides and chemical warfare, yet the current available medical countermeasures are ineffective. The antioxidant, mitoapocynin (MPO), showed limited efficacy orally in rats exposed to an OP. Thus, our research explores three nanoparticle (NP) formulations (CPH:SA, CPTEG:CPH, and a cocktail of the two) as an alternative form of drug delivery for treatment following OP poisoning. The objective of this study was to evaluate NP toxicity when administered intramuscularly in the rat spleen and lung, which is crucial for considering the biocompatibility and bioavailability of NP use in vivo. Results reveal that NP treatment did not alter initial body weight significantly, but acute mononuclear infiltration occurred in the spleen and lung, with CPH:SA showing fewer side effects. Following OP-exposure, acute effects were observed in the lungs and spleen. The conclusions highlight the need for further investigation into NP formulations, dosing regimens, and the route of administration. This project underscores the complexity of NP interactions in the vital internal organs, emphasizing the necessity for future research to draw meaningful conclusions and consider the lasting effects of MPO-NP treatment. Acknowledgment: This project is supported by the by the National Institute of Health/NINDS through the CounterACT Program (R21 NS120916).
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Morgan Kazmierczak – Animal Science
Good Boys with Bad Habits
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Project Advisor(s): Cheryl Morris | Advisor(s): Jennifer Bundy
Abstract: Good Boys with Bad Habits is a booklet designed to educate and promote the Ace Project to prospective students at Iowa State University. Founded by Dr. Cheryl Morris, the Ace Project is an independent study course that teaches students about canine behavior and training through hands-on experience with shelter dogs, agility dogs, service dogs in training, and privately owned dogs. The booklet walks students through a semester in the Ace Project and is broken down into several short chapters. Each chapter discusses a topic, such as a common milestone or challenge faced by students in the Ace Project. While beneficial for all prospective students, a specific emphasis has been placed on supporting students who choose to foster a dog through the course. The ultimate goal of the booklet is to foster the growth of the Ace Project while simultaneously preventing attrition due to ill-prepared student participants. Information for the booklet was collected through Ace Project class resources, online articles, survey responses from current Ace Project participants, and the author’s personal experiences. The final project was created using Shutterfly.
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Julie Lennon – Global Resource Systems, Environmental Science (LAS)
Evaluating the Potential to Reduce Soil Transport at Strategic Flowpaths in western St. John USVI, Using Biological Management Processes
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Project Advisor(s): Emily Zimmerman | Advisor(s): Amanda Chung, Richard Williams
Abstract: Mountainous terrain and high precipitation results in sediment loss from runoff. Sediment settles onto algae, preventing photosynthesis and harming corals, with which the algae are symbionts. Implementing biological management processes (BMPs) where water intersects with roads could reduce the amount of sediment transported downhill. The objectives of this study were (1) Validate points obtained from the model by observing flowpaths, (2) Record structures, vegetation, and topography at each point, (3) Assess potential to implement vegetative BMPs at each point, and (4) Review literature to evaluate types of BMPs. A model adapted for St. John identified 100 probable intersections, which were verified and surveyed to determine suitability for BMPs. Data on accessibility, pavement type, visibility and evidence of flowpath, existing structures, vegetative cover, and elevation were collected at each point. A literature search was conducted to review vegetative BMP techniques and plant species. 68 points had visible evidence of a flowpath. Relatively flat terrain and no existing management structures were selected as criteria for determining priority locations to implement BMPs. 24 articles discussed vegetative techniques, 14 articles suggested plant species, and 8 articles listed groups of plants. Implementation of recommended techniques and plants at priority locations could quantify their reduction potential.
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Rebecca Lynn – Forestry
Wildlife Report on Restored Riparian Buffer in the Bear Creek Watershed
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Project Advisor(s): Richard Schultz | Advisor(s): Richard Schultz
Abstract: During the 1990s a riparian area of the Bear Creek Watershed near Roland, Iowa was restored. Since the restoration project, little research has been conducted to examine the benefits that the project had on wildlife. This study analyzed the current bird, aquatic insect, and mammal species that have returned to the restored section of Bear Creek. The aim of this study was to determine the abundance of species using the riparian area and not the population numbers of each species. The location where Bear Creek joins the Skunk River was used as the control in this experiment. The control has historically been under native vegetation and has not been farmed. The data gathered from this study reflects the habitat quality of Bear Creek and the riparian area surrounding the creek.
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Brenna Mathers – Genetics (AGLS)
Drosophila PTTH Mutants to Analyze Aging and Muscle Function
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Project Advisor(s): Ping Kang | Advisor(s): Alison Esser
Abstract: Prothoracicotropic Hormone (PTTH) is a growth factor that regulates the developmental timing and aging of many insects, including Drosophila. Mutants of the gene encoding the PTTH protein have been shown to impact the larval stages, oenocyte development, and immune response. Underexpression of the gene has been shown to increase lifespan and vice versa. In this study, I’m analyzing the effect of a PTTH knockout mutant where Red Fluorescent Protein (RFP) has been inserted into the gene to disrupt it. Using a climbing assay completed over six weeks, I compared homozygous wild-type flies, homozygous mutant flies, and both crosses of the heterozygous, where we controlled which parent the mutant allele came from. My objective is to determine whether or not PTTH has a similar impact on muscle development and degradation as it does on other developmental processes and lifespan. Understanding the scope of biological processes that PTTH has on insects, both in the early stages and throughout adulthood, can be a useful tool in deciphering similar growth hormones found in humans.
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Sierra McCoy – Forestry
funded by University Honors Grant
Campus Trees of Iowa State
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Project Advisor(s): William Beck | Advisor(s): Miranda Curzon
Abstract: Trees supply many benefits to people, with the trees on campus serving students, faculty, and the public. Campus Trees of Iowa State was created to teach passersby the names, history, and importance of these trees. By forging an interest and appreciation for the trees on campus, there’s hope that a solid foundation of knowledge is developed and will act as a gateway to appreciating urban forests everywhere, as they are often taken for granted. Trees were chosen based on historical significance, aesthetic value, and location, and a mix of species were included to showcase a diverse variety of arboreal concepts. Physical signage was designed and placed for most of the trees involved in the project, and online points were created for every tree. Information for each tree included common name, scientific name, any historical significance of the specific individual or group of trees, and information relating to the genus or species.
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Shannon Morgan – Microbiology
Exploring the Relationship Between miRNAs and Synbiotics as Therapeutic Potential for Dogs with Chronic Inflammatory Enteropathy
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Project Advisor(s): Albert Jergens | Advisor(s): Joan Cunnick
Abstract: Canine inflammatory bowel disease (IBD) is a chronic enteropathy characterized by vomiting, diarrhea, and weight loss due to intestinal inflammation. IBD is a multifactorial disease triggered by the interaction of environmental factors, mucosal immunity, and host genetics. The use of 2D immortalized cell lines or animal models that have limited translatability to human IBD have fostered the development of intestinal epithelial 3D cell (IEC) cultures called intestinal organoids, lab-grown from biopsy-obtained stem cells. Our focus was on ileal-derived enteroids due to their relevance to human IBD, our goal being to define phenotypic and functional changes between healthy and IBD dogs. After cultivating organoids, RNA in-situ hybridization was performed to target specific epithelial cell surface markers, as well as immunohistochemistry staining. Results followed expectations regarding IBD inflammation. LGR5 stem cell expression increased in IBD dogs, showing greater cell turnover, and ALP downregulation shows decreased absorptive ability of the epithelia. A decrease in intestinal barrier protein indicates leaky gut, and less mucin coincides with intestinal barrier dysfunction and a problem with host immune defenses. These results prove promising in using organoids for IBD research where tissues don’t suffice, and aid in the understanding of how epithelial physiology contributes to IBD development.
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Jenna Plotzke – Global Resource Systems, Environmental Science (AGLS)
Drainage District Main Improvements in Iowa
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Project Advisor(s): Matthew Helmers | Advisor(s): Amanda Chung, Richard Williams
Abstract: Iowa agriculture within the Des Moines Lobe (west/central Iowa) is dependent upon drainage systems to keep the corn and soybean fields producing year after year due to previous glaciation. In the early 1900s, drainage systems became a popular and feasible way for farmers to remove the excess water from these fields. There is a concern that there are drainage systems that are currently in place across the Des Moines Lobe that are under-capacity and the fields will not be drained properly to keep productivity up. This research examined recent drainage main improvement projects to evaluate the percent of projects that move forward, the cost of these projects, and the old and new drainage coefficient. The work found that most drainage main improvement projects have moved forward and the average cost per acre is $800.
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Jordan Sanders – Animal Ecology
Isolation of Porcine Astrovirus 4
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Project Advisor(s): Michael Rahe | Advisor(s): John Burnett
Abstract: Porcine astrovirus 4 (PoAstV4) has been strongly associated with respiratory disease in young pigs. The objective of this project was to isolate PoAstV4 to help determine if the virus causes respiratory disease. PK15 and Caco2 cells were the two cell lines that were identified as possible permissive cells for PoAstV4. In brief, cell cultures were inoculated with viral suspension from infected samples and incubated for 2-3 days. RT-PCR was employed to monitor replication of the virus in cell cultures. The results of isolation attempts indicated little to no viral replication or cell death in tested cell lines. This could be for multiple reasons: it could take multiple inoculations to get the needed amount of cell death, the virus couldn’t grow in those cells, or the virus may require an unknown enzyme to infect. Infected tissue homogenate was also prepared for a porcine challenge study. The results of the study showed that pigs were infected with PoAstV4 before reaching the isolation facility. This was yet another example associating PoAstV4 with respiratory disease. An upcoming study in cesarean derived and colostrum deprived pigs will test if PoAstV4 is a cause of respiratory disease.
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Sarah Schroeder – Food Science (AGLS), Global Resource Systems
An Evaluation of the European Union’s Farm to Fork Strategy: Formation, Feedback, and Moving Forward
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Project Advisor(s): Emily Zimmerman | Advisor(s): Melha Mellata, Amanda Chung
Abstract: The European Union has taken steps to promote long-term sustainable development through the Farm to Fork Strategy. The Farm to Fork Strategy is of global significance, as transitions within the European Union will impact global trade and supply chains and lay the groundwork for future food systems legislation. The research objectives were to synthesize stakeholder feedback on the proposal for a legislative framework for sustainable food systems and Organic Action Plan and form policy recommendations. Content analysis was carried out of secondary, qualitative data found in literature from which common themes were recorded, synthesized, and used to meet objectives. Review of feedback on the proposed sustainable food systems initiative showed support for a comprehensive framework legislation. Stakeholders expressed desire for a common definition of sustainability and the adoption of a true-cost accounting approach. The European Union should move forward with forming a comprehensive framework legislation. For the Organic Action Plan, two stakeholder-identified obstacles to an increased organic food system were insufficient financial incentives and low consumer awareness, both of which are addressed in the adopted Organic Action Plan. Actions should be taken to enhance the economic feasibility of producing and consuming organic products.
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Christina Speicher – Animal Science
Characterization of the Physiochemical Properties of Plant-Based Bigels in Comparison to Animal-Based Fats
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Project Advisor(s): Rodrigo Tarte | Advisor(s): Sherrlyn Olsen
Abstract: Over the years, replacement of animal fat in course ground products has been challenging. Recently, biphasic gel (BPG) systems have been shown to be a potential solution. However, to have universal application, they should be made from plant-sourced ingredients. In this study, we developed and characterized two biphasic gels consisting of an oleogel (OG) phase (92.5% high-oleic soybean oil, 7.5% rice bran wax) and a hydrogel (HG) phase (96% water, 2% soy protein isolate, 2% -carrageenan), combined in 5:5 and 4:6 OG:HG ratios. The BPGs were stored at 2–3°C for 28 days and analyzed every 7 days for color, Texture Profile Analysis (TPA), lipid oxidation, and pH. The color of 5:5 BPG was lighter than 4:6 BPG (P < .05), a difference that is unlikely to be of practical importance. No other color differences were observed. For texture, 4:6 BPG was more resilient than 5:5 BPG. Both gels were softer on day 7 and regained hardness thereafter. pH and lipid oxidation were unaffected by BPG formulation and storage time. Our results indicate that the two gels were comparable to each other in all attributes measured, and that they remained stable under refrigeration conditions up to 28 days.
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Whitney Winter – Animal Ecology
Validation of AIM 2 Research Utilizing Primer Design
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Project Advisor(s): Chris Tuggle, Anna Johnson | Advisor(s): Stephen Dinsmore
Abstract: The objective of this project is to further validate research done by R. Corbett on Parent of parent-of-origin effect in swine (Sus Scrofa). We selected and designed 20 primers for 18 genes previously determined to express varying levels of heterozygosity in a Single Nucleotide Polymorphism (SNP) site. Each primer was tested with a variation of DNA concentrations in order to minimize the total number of DNA needed in order to perform further testing due to the limited sample volume. We determined that 5 nanogram/microliter was the optimal concentration, halving the amount of DNA that is typically needed. Following PCR testing, each successful primer was used to purify its determined gene of interest utilizing PCR. The samples were then sent to be sequenced and a chromatogram was imaged. We then created new methods that were utilized to determine the peak size of the two nucleotide possibilities in the SNP site. This method involved printing a scaled image of the SNP site and utilizing rulers to measure the height of each peak. The measurements were then placed in a ratio in order to determine the accuracy of Corbett's prior research.